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Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP.

ABSTRACT: Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may influence DGAT1 expression and function. The results described here could be useful in examining the association between the DGAT1 gene and milk traits in buffalo.

SUBMITTER: Raut AA 

PROVIDER: S-EPMC3459411 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP.

Raut Ashwin A AA   Kumar Anil A   Kala Sheo N SN   Chhokar Vinod V   Rana Neeraj N   Beniwal Vikas V   Jaglan Sundeep S   Samuchiwal Sachin K SK   Singh Jitender K JK   Mishra Anamika A  

Genetics and molecular biology 20120713 3

Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single  ...[more]

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