Exploring the DNA-binding specificities of zinc fingers with DNA microarrays.

ABSTRACT: A key step in the regulation of networks that control gene expression is the sequence-specific binding of transcription factors to their DNA recognition sites. A more complete understanding of these DNA-protein interactions will permit a more comprehensive and quantitative mapping of the regulatory pathways within cells, as well as a deeper understanding of the potential functions of individual genes regulated by newly identified DNA-binding sites. Here we describe a DNA microarray-based method to characterize sequence-specific DNA recognition by zinc-finger proteins. A phage display library, prepared by randomizing critical amino acid residues in the second of three fingers of the mouse Zif268 domain, provided a rich source of zinc-finger proteins with variant DNA-binding specificities. M

SUBMITTER: Bulyk ML 

PROVIDER: S-EPMC34639 | biostudies-literature | 2001 Jun

REPOSITORIES: biostudies-literature