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Detection and isolation of dendritic cells using Lewis X-functionalized magnetic nanoparticles.

ABSTRACT: dendritic cell (DC)-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) is a receptor found on DCs that recognizes antigens bearing mannose-rich or fucosylated glycans, including Lewis X (Le(X)). Here, we report the fabrication of magnetic nanoparticles coated with multivalent Le(X) glycans using Cu (I)-catalyzed azide-alkyne cycloaddition. The resulting nanoparticles are selective and biocompatible, serving as a highly efficient tool for DC detection and enrichment.

SUBMITTER: Rouhanifard SH

PROVIDER: S-EPMC3470862 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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Detection and isolation of dendritic cells using Lewis X-functionalized magnetic nanoparticles.

Rouhanifard Sara H SH Xie Ran R Zhang Guoxin G Sun Xiaoming X Chen Xing X Wu Peng P

Biomacromolecules 20120911 10

dendritic cell (DC)-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) is a receptor found on DCs that recognizes antigens bearing mannose-rich or fucosylated glycans, including Lewis X (Le(X)). Here, we report the fabrication of magnetic nanoparticles coated with multivalent Le(X) glycans using Cu (I)-catalyzed azide-alkyne cycloaddition. The resulting nanoparticles are selective and biocompatible, serving as a highly efficient tool for DC detection and enrichment. ...[more]

PMID: 22901307

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