Ontology highlight
ABSTRACT:
SUBMITTER: Sikorski TW
PROVIDER: S-EPMC3471693 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Sikorski Timothy W TW Joo Yoo Jin YJ Ficarro Scott B SB Askenazi Manor M Buratowski Stephen S Marto Jarrod A JA
The Journal of biological chemistry 20120817 42
In-depth characterization of RNA polymerase II preinitiation complexes remains an important and challenging goal. We used quantitative mass spectrometry to explore context-dependent Saccharomyces cerevisiae preinitiation complex formation at the HIS4 promoter reconstituted on naked and chromatinized DNA templates. The transcription activators Gal4-VP16 and Gal4-Gcn4 recruited a limited set of chromatin-related coactivator complexes, namely the chromatin remodeler Swi/Snf and histone acetyltransf ...[more]