Ontology highlight
ABSTRACT:
SUBMITTER: Lehmann L
PROVIDER: S-EPMC3476247 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
The Journal of biological chemistry 20120821 43
Despite the important role of Polycomb in genome-wide silencing, little is known of the specific biochemical mechanism by which it inactivates transcription. Here we address how recombinant Polycomb repressive complex 1 (PRC1) inhibits activated RNA polymerase II preinitiation complex (PIC) assembly using immobilized H3K27-methylated chromatin templates in vitro. Recombinant PRC1 inhibited transcription, but had little effect on binding of the activator as reported previously. In contrast, Media ...[more]