Ontology highlight
ABSTRACT:
SUBMITTER: Murakami K
PROVIDER: S-EPMC4640751 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Murakami Kenji K Tsai Kuang-Lei KL Kalisman Nir N Bushnell David A DA Asturias Francisco J FJ Kornberg Roger D RD
Proceedings of the National Academy of Sciences of the United States of America 20151019 44
The structure of a 33-protein, 1.5-MDa RNA polymerase II preinitiation complex (PIC) was determined by cryo-EM and image processing at a resolution of 6-11 Å. Atomic structures of over 50% of the mass were fitted into the electron density map in a manner consistent with protein-protein cross-links previously identified by mass spectrometry. The resulting model of the PIC confirmed the main conclusions from previous cryo-EM at lower resolution, including the association of promoter DNA only with ...[more]