Ontology highlight
ABSTRACT:
SUBMITTER: Chen HT
PROVIDER: S-EPMC2483787 | biostudies-literature | 2007 Aug
REPOSITORIES: biostudies-literature
Chen Hung-Ta HT Warfield Linda L Hahn Steven S
Nature structural & molecular biology 20070715 8
We incorporated the non-natural photoreactive amino acid p-benzoyl-L-phenylalanine (Bpa) into the RNA polymerase II (Pol II) surface surrounding the central cleft formed by the Rpb1 and Rpb2 subunits. Photo-cross-linking of preinitiation complexes (PICs) with these Pol II derivatives and hydroxyl-radical cleavage assays revealed that the TFIIF dimerization domain interacts with the Rpb2 lobe and protrusion domains adjacent to Rpb9, while TFIIE cross-links to the Rpb1 clamp domain on the opposite ...[more]