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Overcoming transcription activator-like effector (TALE) DNA binding domain sensitivity to cytosine methylation.

ABSTRACT: Within the past 2 years, transcription activator-like effector (TALE) DNA binding domains have emerged as the new generation of engineerable platform for production of custom DNA binding domains. However, their recently described sensitivity to cytosine methylation represents a major bottleneck for genome engineering applications. Using a combination of biochemical, structural, and cellular approaches, we were able to identify the molecular basis of such sensitivity and propose a simple, drug-free, and universal method to overcome it.

SUBMITTER: Valton J

PROVIDER: S-EPMC3493886 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Overcoming transcription activator-like effector (TALE) DNA binding domain sensitivity to cytosine methylation.

Valton Julien J Dupuy Aurélie A Daboussi Fayza F Thomas Séverine S Maréchal Alan A Macmaster Rachel R Melliand Kevin K Juillerat Alexandre A Duchateau Philippe P

The Journal of biological chemistry 20120926 46

Within the past 2 years, transcription activator-like effector (TALE) DNA binding domains have emerged as the new generation of engineerable platform for production of custom DNA binding domains. However, their recently described sensitivity to cytosine methylation represents a major bottleneck for genome engineering applications. Using a combination of biochemical, structural, and cellular approaches, we were able to identify the molecular basis of such sensitivity and propose a simple, drug-fr ...[more]

PMID: 23019344

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