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Co-transcriptional assembly of chemically modified RNA nanoparticles functionalized with siRNAs.

ABSTRACT: We report a generalized methodology for the one-pot production of chemically modified functional RNA nanoparticles during in vitro transcription with T7 RNA polymerase. The efficiency of incorporation of 2'-fluoro-dNTP in the transcripts by the wild type T7 RNA polymerase dramatically increases in the presence of manganese ions, resulting in a high-yield production of chemically modified RNA nanoparticles functionalized with siRNAs that are resistant to nucleases from human blood serum. Moreover, the unpurified transcription mixture can be used for functional ex vivo pilot experiments.

SUBMITTER: Afonin KA

PROVIDER: S-EPMC3498980 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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Co-transcriptional assembly of chemically modified RNA nanoparticles functionalized with siRNAs.

Afonin Kirill A KA Kireeva Maria M Grabow Wade W WW Kashlev Mikhail M Jaeger Luc L Shapiro Bruce A BA

Nano letters 20120927 10

We report a generalized methodology for the one-pot production of chemically modified functional RNA nanoparticles during in vitro transcription with T7 RNA polymerase. The efficiency of incorporation of 2'-fluoro-dNTP in the transcripts by the wild type T7 RNA polymerase dramatically increases in the presence of manganese ions, resulting in a high-yield production of chemically modified RNA nanoparticles functionalized with siRNAs that are resistant to nucleases from human blood serum. Moreover ...[more]

PMID: 23016824

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