Ontology highlight
ABSTRACT:
SUBMITTER: Afonin KA
PROVIDER: S-EPMC3498981 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Afonin Kirill A KA Grabow Wade W WW Walker Faye M FM Bindewald Eckart E Dobrovolskaia Marina A MA Shapiro Bruce A BA Jaeger Luc L
Nature protocols 20111201 12
Individual genes can be targeted with siRNAs. The use of nucleic acid nanoparticles (NPs) is a convenient method for delivering combinations of specific siRNAs in an organized and programmable manner. We present three assembly protocols to produce two different types of RNA self-assembling functional NPs using processes that are fully automatable. These NPs are engineered based on two complementary nanoscaffold designs (nanoring and nanocube), which serve as carriers of multiple siRNAs. The NPs ...[more]