Ontology highlight
ABSTRACT:
SUBMITTER: Handlogten MW
PROVIDER: S-EPMC3500431 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Handlogten Michael W MW Kiziltepe Tanyel T Alves Nathan J NJ Bilgicer Basar B
ACS chemical biology 20120821 11
This study describes the design of a well-defined homotetravalent synthetic allergen (HTA) system to investigate the effect of hapten-IgE interactions on mast cell degranulation. A library of DNP variants with varying affinities for IgE(DNP) was generated (K(d) from 8.1 nM to 9.2 μM), and 8 HTAs spanning this range were synthesized via conjugation of each DNP variant to the tetravalent scaffold. HTAs with hapten K(d) < 235 nM stimulated degranulation following a bell-shaped dose response curve w ...[more]