Unknown

Dataset Information

0

Secreted Vago restricts West Nile virus infection in Culex mosquito cells by activating the Jak-STAT pathway.


ABSTRACT: Although West Nile virus (WNV) and other arthropod-borne viruses are a major public health problem, the mechanisms of antiviral immunity in mosquitoes are poorly understood. Dicer-2, responsible for the RNAi-mediated response through the C-terminal RNase-III domain, also contains an N-terminal DExD/H-box helicase domain similar to mammalian RIG-I/MDA5 which, in Drosophila, was found to be required for activation of an antiviral gene, Vago. Here we show that the Culex orthologue of Vago (CxVago) is up-regulated in response to WNV infection in a Dicer-2-dependent manner. Further, our data show that CxVago is a secreted peptide that restricts WNV infection by activation of the Jak-STAT pathway. Thus, Vago appears to function as an IFN-like antiviral cytokine in mosquitoes.

SUBMITTER: Paradkar PN 

PROVIDER: S-EPMC3503207 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Secreted Vago restricts West Nile virus infection in Culex mosquito cells by activating the Jak-STAT pathway.

Paradkar Prasad N PN   Trinidad Lee L   Voysey Rhonda R   Duchemin Jean-Bernard JB   Walker Peter J PJ  

Proceedings of the National Academy of Sciences of the United States of America 20121001 46


Although West Nile virus (WNV) and other arthropod-borne viruses are a major public health problem, the mechanisms of antiviral immunity in mosquitoes are poorly understood. Dicer-2, responsible for the RNAi-mediated response through the C-terminal RNase-III domain, also contains an N-terminal DExD/H-box helicase domain similar to mammalian RIG-I/MDA5 which, in Drosophila, was found to be required for activation of an antiviral gene, Vago. Here we show that the Culex orthologue of Vago (CxVago)  ...[more]

Similar Datasets

| S-EPMC4091933 | biostudies-literature
| S-EPMC2823692 | biostudies-literature
| S-EPMC6466260 | biostudies-literature
| S-EPMC3100213 | biostudies-literature
| S-EPMC3151626 | biostudies-literature
| S-EPMC3433782 | biostudies-literature
2015-10-01 | GSE60229 | GEO
| S-EPMC9502061 | biostudies-literature
| S-EPMC3249650 | biostudies-literature
| S-EPMC3241966 | biostudies-literature