Dataset Information

Fatty acids regulate endothelial lipase and inflammatory markers in macrophages and in mouse aorta: a role for PPAR?.

ABSTRACT: Macrophage endothelial lipase (EL) is associated with increased atherosclerosis and inflammation. Because of their anti-inflammatory properties we hypothesized that n-3 fatty acids, in contrast to saturated fatty acids, would lower macrophages and arterial EL and inflammatory markers.Murine J774 and peritoneal macrophages were incubated with eicosapentaenoic acid or palmitic acid in the presence or absence of lipopolysaccaride (LPS). LPS increased EL mRNA and protein. Palmitic acid alone or with LPS dose-dependently increased EL mRNA and protein. In contrast, eicosapentaenoic acid dose-dependently abrogated effects of LPS or palmitic acid on increasing EL expression. EL expression closely linked to peroxisome proliferator activated receptor (PPAR)? expression. Eicosapentaenoic acid blocked rosiglitazone (a PPAR? agonist)-mediated EL activation and GW9662 (a PPAR? antagonist)-blocked palmitic acid-mediated EL stimulation. Eicosapentaenoic acid alone or with LPS blunted LPS-mediated stimulation of macrophage proinflammatory interleukin-6, interleukin-12p40, and toll-like receptor-4 mRNA and increased anti-inflammatory interleukin-10 and mannose receptor mRNA. In vivo studies in low density lipoprotein receptor knockout mice showed that high saturated fat rich diets, but not n-3 diets, increased arterial EL, PPAR?, and proinflammatory cytokine mRNA.n-3 fatty acids, in contrast to saturated fatty acids, decrease EL in parallel with modulating pro- and anti-inflammatory markers, and these effects on EL link to PPAR?.

SUBMITTER: Jung UJ

PROVIDER: S-EPMC3512203 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Fatty acids regulate endothelial lipase and inflammatory markers in macrophages and in mouse aorta: a role for PPARγ.

Jung Un Ju UJ Torrejon Claudia C Chang Chuchun L CL Hamai Hiroko H Worgall Tilla S TS Deckelbaum Richard J RJ

Arteriosclerosis, thrombosis, and vascular biology 20121004 12

<h4>Objective</h4>Macrophage endothelial lipase (EL) is associated with increased atherosclerosis and inflammation. Because of their anti-inflammatory properties we hypothesized that n-3 fatty acids, in contrast to saturated fatty acids, would lower macrophages and arterial EL and inflammatory markers.<h4>Methods and results</h4>Murine J774 and peritoneal macrophages were incubated with eicosapentaenoic acid or palmitic acid in the presence or absence of lipopolysaccaride (LPS). LPS increased EL ...[more]

PMID: 23042820

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Dataset Information

Fatty acids regulate endothelial lipase and inflammatory markers in macrophages and in mouse aorta: a role for PPAR?.

Publications

Fatty acids regulate endothelial lipase and inflammatory markers in macrophages and in mouse aorta: a role for PPARγ.

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