Ontology highlight
ABSTRACT:
SUBMITTER: Tyler RE
PROVIDER: S-EPMC3521676 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Tyler Ryan E RE Pearce Margaret M P MM Shaler Thomas A TA Olzmann James A JA Greenblatt Ethan J EJ Kopito Ron R RR
Molecular biology of the cell 20121024 24
Degradation of folding- or assembly-defective proteins by the endoplasmic reticulum-associated degradation (ERAD) ubiquitin ligase, Hrd1, is facilitated by a process that involves recognition of demannosylated N-glycans by the lectin OS-9/XTP3-B via the adaptor protein SEL1L. Most of our knowledge of the machinery that commits proteins to this fate in metazoans comes from studies of overexpressed mutant proteins in heterologous cells. In this study, we used mass spectrometry to identify core-gly ...[more]