Ontology highlight
ABSTRACT:
SUBMITTER: Preston GM
PROVIDER: S-EPMC5912696 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Preston G Michael GM Guerriero Christopher J CJ Metzger Meredith B MB Michaelis Susan S Brodsky Jeffrey L JL
Molecular cell 20180401 2
Misfolded proteins in the endoplasmic reticulum (ER) are destroyed by ER-associated degradation (ERAD). Although the retrotranslocation of misfolded proteins from the ER has been reconstituted, how a polypeptide is initially selected for ERAD remains poorly defined. To address this question while controlling for the diverse nature of ERAD substrates, we constructed a series of truncations in a single ER-tethered domain. We observed that the truncated proteins exhibited variable degradation rates ...[more]