Ontology highlight
ABSTRACT:
SUBMITTER: Dai K
PROVIDER: S-EPMC3541276 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Dai Kun K Liao Shanhui S Zhang Jiahai J Zhang Xuecheng X Tu Xiaoming X
PloS one 20130109 1
The ADF/cofilin family has been characterized as a group of actin-binding proteins critical for controlling the assembly of actin within the cells. In this study, the solution structure of the ADF/cofilin from Trypanosoma brucei (TbCof) was determined by NMR spectroscopy. TbCof adopts the conserved ADF/cofilin fold with a central β-sheet composed of six β-strands surrounded by five α-helices. Isothermal titration calorimetry experiments denoted a submicromolar affinity between TbCof and G-actin, ...[more]