Ontology highlight
ABSTRACT:
SUBMITTER: Plank M
PROVIDER: S-EPMC3546318 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Plank Michael M Hu Guang G Silva A Sofia AS Wood Shona H SH Hesketh Emily E EE Janssens Georges G Macedo André A de Magalhães João Pedro JP Church George M GM
Scientific reports 20130116
Large-scale RNAi-based screens are a major technology, but require adequate prioritization and validation of candidate genes from the primary screen. In this work, we performed a large-scale pooled shRNA screen in mouse embryonic stem cells (ESCs) to discover genes associated with oxidative stress resistance and found several candidates. We then developed a bioinformatics pipeline to prioritize these candidates incorporating effect sizes, functional enrichment analysis, interaction networks and ...[more]