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An expanded palette of genetically encoded Ca²? indicators.

ABSTRACT: Engineered fluorescent protein (FP) chimeras that modulate their fluorescence in response to changes in calcium ion (Ca(2+)) concentration are powerful tools for visualizing intracellular signaling activity. However, despite a decade of availability, the palette of single FP-based Ca(2+) indicators has remained limited to a single green hue. We have expanded this palette by developing blue, improved green, and red intensiometric indicators, as well as an emission ratiometric indicator with an 11,000% ratio change. This series enables improved single-color Ca(2+) imaging in neurons and transgenic Caenorhabditis elegans. In HeLa cells, Ca(2+) was imaged in three subcellular compartments, and, in conjunction with a cyan FP-yellow FP-based indicator, Ca(2+) and adenosine 5'-triphosphate were simultaneously imaged. This palette of indicators paints the way to a colorful new era of Ca(2+) imaging.

SUBMITTER: Zhao Y

PROVIDER: S-EPMC3560286 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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An expanded palette of genetically encoded Ca²⁺ indicators.

Zhao Yongxin Y Araki Satoko S Wu Jiahui J Teramoto Takayuki T Chang Yu-Fen YF Nakano Masahiro M Abdelfattah Ahmed S AS Fujiwara Manabi M Ishihara Takeshi T Nagai Takeharu T Campbell Robert E RE

Science (New York, N.Y.) 20110908 6051

Engineered fluorescent protein (FP) chimeras that modulate their fluorescence in response to changes in calcium ion (Ca(2+)) concentration are powerful tools for visualizing intracellular signaling activity. However, despite a decade of availability, the palette of single FP-based Ca(2+) indicators has remained limited to a single green hue. We have expanded this palette by developing blue, improved green, and red intensiometric indicators, as well as an emission ratiometric indicator with an 11 ...[more]

PMID: 21903779

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