Ontology highlight
ABSTRACT:
SUBMITTER: Yeeles JT
PROVIDER: S-EPMC3593629 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Yeeles Joseph T P JT Marians Kenneth J KJ
Science (New York, N.Y.) 20111001 6053
The Escherichia coli DNA replication machinery must frequently overcome template lesions under normal growth conditions. Yet, the outcome of a collision between the replisome and a leading-strand template lesion remains poorly understood. Here, we demonstrate that a single, site-specific, cyclobutane pyrimidine dimer leading-strand template lesion provides only a transient block to fork progression in vitro. The replisome remains stably associated with the fork after collision with the lesion. L ...[more]