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Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization.

ABSTRACT: Membrane protein manipulation is a challenging task owing to limited tertiary and quaternary structural stability once the protein has been removed from a lipid bilayer. Such instability can be overcome by embedding membrane proteins in detergent micelles formed from amphiphiles with carefully tuned properties. This study introduces a class of easy-to-synthesize amphiphiles, which are designated CGT (Chae's Glyco-Triton) detergents. Some of the agents are well suited for membrane protein solubilization and stabilization.

SUBMITTER: Chae PS

PROVIDER: S-EPMC3593792 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization.

Chae Pil Seok PS Wander Marc J MJ Cho Kyung Ho KH Laible Philip D PD Gellman Samuel H SH

Molecular bioSystems 20130401 4

Membrane protein manipulation is a challenging task owing to limited tertiary and quaternary structural stability once the protein has been removed from a lipid bilayer. Such instability can be overcome by embedding membrane proteins in detergent micelles formed from amphiphiles with carefully tuned properties. This study introduces a class of easy-to-synthesize amphiphiles, which are designated CGT (Chae's Glyco-Triton) detergents. Some of the agents are well suited for membrane protein solubil ...[more]

PMID: 23377371

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