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Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies).

ABSTRACT: A finely tuned balance of translation, storage and decay of mRNAs (mRNAs) is important for the regulation of gene expression. In eukaryotic cells, this takes place in dynamic cytoplasmic RNA-protein granules termed Processing bodies (P-bodies). In this study, by using immunoelectron tomography, 3D modeling and template matching, we analyze the size and the organization of the polysomes in the vicinity of human P-bodies. Our results show the presence of several polysomes that are compatible with a translational activity around P-bodies. Therefore, movement of mRNAs between polysomes and P-bodies can take place when the two compartments are in close contact. The presence of initiation factors in the proximity of P-bodies also suggests that translation of mRNAs can resume at the periphery of these granules.

SUBMITTER: Cougot N

PROVIDER: S-EPMC3594289 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies).

Cougot Nicolas N Molza Anne-Elisabeth AE Giudice Emmanuel E Cavalier Annie A Thomas Daniel D Gillet Reynald R

RNA biology 20130116 2

A finely tuned balance of translation, storage and decay of mRNAs (mRNAs) is important for the regulation of gene expression. In eukaryotic cells, this takes place in dynamic cytoplasmic RNA-protein granules termed Processing bodies (P-bodies). In this study, by using immunoelectron tomography, 3D modeling and template matching, we analyze the size and the organization of the polysomes in the vicinity of human P-bodies. Our results show the presence of several polysomes that are compatible with ...[more]

PMID: 23324601

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Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies.

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Project description:This SuperSeries is composed of the SubSeries listed below.

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