Ontology highlight
ABSTRACT:
SUBMITTER: Bowers PM
PROVIDER: S-EPMC3597809 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
The Journal of biological chemistry 20130125 11
A method for simultaneous humanization and affinity maturation of monoclonal antibodies has been developed using heavy chain complementarity-determining region (CDR) 3 grafting combined with somatic hypermutation in vitro. To minimize the amount of murine antibody-derived antibody sequence used during humanization, only the CDR3 region from a murine antibody that recognizes the cytokine hβNGF was grafted into a nonhomologous human germ line V region. The resulting CDR3-grafted HC was paired with ...[more]