Ontology highlight
ABSTRACT:
SUBMITTER: Lee CV
PROVIDER: S-EPMC4011906 | biostudies-literature | 2014 May-Jun
REPOSITORIES: biostudies-literature
Lee Chingwei V CV Koenig Patrick P Fuh Germaine G
mAbs 20140311 3
A mono-specific antibody may recruit a second antigen binding specificity, thus converting to a dual-specific Two-in-One antibody through mutation at the light chain complementarity-determining regions (CDRs). It is, however, unknown whether mutation at the heavy chain CDRs may evolve such dual specificity. Herein, we examined the CDRs of a humanized interleukin 4 (IL4) antibody using alanine scanning and structural modeling, designed libraries of mutants in regions that tolerate mutation, and i ...[more]