Ontology highlight
ABSTRACT:
SUBMITTER: Schumacher FF
PROVIDER: S-EPMC3605607 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Scientific reports 20130101
A major obstacle to the efficient production of antibody conjugates for therapy and diagnosis is the non-ideal performance of commonly used chemical methods for the attachment of effector-molecules to the antibody of interest. Here we demonstrate that this limitation can be simply addressed using 3,4-substituted maleimides to bridge and thus functionalize disulfide bonds to generate homogeneous antibody conjugates. This one-step conjugation reaction is fast, site-specific, quantitative and gener ...[more]