Ontology highlight
ABSTRACT:
SUBMITTER: Lee HW
PROVIDER: S-EPMC3614353 | biostudies-literature | 2007 Jun
REPOSITORIES: biostudies-literature
Lee Hyun-Wook HW Lee Hae-Jung HJ Hong Chong-mu CM Baker David J DJ Bhatia Ravi R O'Connor Timothy R TR
Analytical biochemistry 20070321 2
We introduce a method to follow DNA repair that is suitable for both clinical and laboratory samples. An episomal construct with a unique 8-oxoguanine (8-oxoG) base at a defined position was prepared in vitro using single-stranded phage harboring a 678-bp tract from exons 5 to 9 of the human P53 gene. Mixing curve experiments showed that the real-time PCR method has a linear response to damage, suggesting that it is useful for DNA repair studies. The episomal construct with a unique 8-oxoG base ...[more]