Ontology highlight
ABSTRACT:
SUBMITTER: van Kan M
PROVIDER: S-EPMC6906414 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
van Kan Maia M Burns Kathryn E KE Browett Peter P Helsby Nuala A NA
Scientific reports 20191211 1
Inter-individual differences in DNA adduct formation and repair influence the response to melphalan treatment, however, further clinical investigation of this variability requires a logistically feasible and reproducible bioassay. Our improved fluorescence-based QPCR-block assay is robust, has good precision, and improved throughput. It also incorporates direct PCR amplification from melphalan exposed PBMC using commercially available blood tubes and extraction kits to maximise the utility of th ...[more]