Lysine overproducing Corynebacterium glutamicum is characterized by a robust linear combination of two optimal phenotypic states.

ABSTRACT: A homoserine auxotroph strain of Corynebacterium glutamicum accumulates storage compound trehalose with lysine when limited by growth. Industrially lysine is produced from C. glutamicum through aspartate biosynthetic pathway, where enzymatic activity of aspartate kinase is allosterically controlled by the concerted feedback inhibition of threonine plus lysine. Ample threonine in the medium supports growth and inhibits lysine production (phenotype-I) and its complete absence leads to inhibition of growth in addition to accumulating lysine and trehalose (phenotype-II). In this work, we demonstrate that as threonine concentration becomes limiting, metabolic state of the cell shifts from maximizing growth (phenotype-I) to maximizing trehalose phenotype (phenotype-II) in a highly sensitive mann

SUBMITTER: Rajvanshi M 

PROVIDER: S-EPMC3641285 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature