PKC? and PKC? cooperate functionally in CD3-induced de novo IL-2 mRNA transcription.

ABSTRACT: The physiological functions of PKC? and PKC? isotypes downstream of the antigen receptor have been defined in CD3(+) T cells. In contrast, no function of the second conventional PKC member, PKC?, has been described yet in T cell antigen receptor signalling. To investigate the hypothesis that both conventional PKC? and PKC? isotypes may have overlapping functions in T cell activation signalling, we generated mice that lacked the genes for both isotypes. We found that PKC?(-/-)/?(-/-) animals are viable, live normal life spans and display normal T cell development. However, these animals possess additive defects in T cell responses in comparison to animals that carry single mutations in these genes. Our studies demonstrate that the activities of PKC? and PKC? converge to regulate IL-2 cytokine responses in anti-CD3 stimulated primary mouse T cells. Here, we present genetic evidence that PKC? and PKC? cooperate in IL-2 transcriptional transactivation in primary mouse T cells independently of the actions of PKC?.

SUBMITTER: Lutz-Nicoladoni C 

PROVIDER: S-EPMC3641392 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature