Structural visualization of de novo initiation of RNA polymerase II transcription
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ABSTRACT:
SUBMITTER: Stefan Steimle
PROVIDER: EMPIAR-10865 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural visualization of de novo transcription initiation by Saccharomyces cerevisiae RNA polymerase II.
Yang Chun C Fujiwara Rina R Kim Hee Jong HJ Basnet Pratik P Zhu Yunye Y Gorbea Colón Jose J JJ Steimle Stefan S Garcia Benjamin A BA Kaplan Craig D CD Murakami Kenji K
Molecular cell 20220119 3
Previous structural studies of the initiation-elongation transition of RNA polymerase II (pol II) transcription have relied on the use of synthetic oligonucleotides, often artificially discontinuous to capture pol II in the initiating state. Here, we report multiple structures of initiation complexes converted de novo from a 33-subunit yeast pre-initiation complex (PIC) through catalytic activities and subsequently stalled at different template positions. We determine that PICs in the initially ...[more]
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