Ontology highlight
ABSTRACT:
SUBMITTER: Min D
PROVIDER: S-EPMC3644077 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Min Duyoung D Kim Kipom K Hyeon Changbong C Cho Yong Hoon YH Shin Yeon-Kyun YK Yoon Tae-Young TY
Nature communications 20130101
Formation of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex provides mechanical thrust for membrane fusion, but its molecular mechanism is still unclear. Here using magnetic tweezers, we observe mechanical responses of a single neuronal SNARE complex under constant pulling force. Single SNARE complexes may be unzipped with 34 pN force. When rezipping is induced by lowering the force to 11 pN, only a partially assembled state results, with the C-terminal ...[more]