Dataset Information

Intestinal microbiota in healthy U.S. young children and adults--a high throughput microarray analysis.

ABSTRACT:

Unlabelled

It is generally believed that the infant's microbiota is established during the first 1-2 years of life. However, there is scarce data on its characterization and its comparison to the adult-like microbiota in consecutive years.

Aim

To characterize and compare the intestinal microbiota in healthy young children (1-4 years) and healthy adults from the North Carolina region in the U.S. using high-throughput bacterial phylogenetic microarray analysis.

Methods

Detailed characterization and comparison of the intestinal microbiota of healthy children aged 1-4 years old (n = 28) and healthy adults of 21-60 years (n = 23) was carried out using the Human Intestinal Tract Chip (HITChip) phylogenetic microarray targeting the V1 and V6 regions of 16S rRNA and quantitative PCR.

Results

The HITChip microarray data indicate that Actinobacteria, Bacilli, Clostridium cluster IV and Bacteroidetes are the predominant phylum-like groups that exhibit differences between young children and adults. The phylum-like group Clostridium cluster XIVa was equally predominant in young children and adults and is thus considered to be established at an early age. The genus-like level show significant 3.6 fold (higher or lower) differences in the abundance of 26 genera between young children and adults. Young U.S. children have a significantly 3.5-fold higher abundance of Bifidobacterium species than the adults from the same location. However, the microbiota of young children is less diverse than that of adults.

Conclusions

We show that the establishment of an adult-like intestinal microbiota occurs at a later age than previously reported. Characterizing the microbiota and its development in the early years of life may help identify 'windows of opportunity' for interventional strategies that may promote health and prevent or mitigate disease processes.

SUBMITTER: Ringel-Kulka T

PROVIDER: S-EPMC3662718 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Publications

Intestinal microbiota in healthy U.S. young children and adults--a high throughput microarray analysis.

Ringel-Kulka Tamar T Cheng Jing J Ringel Yehuda Y Salojärvi Jarkko J Carroll Ian I Palva Airi A de Vos Willem M WM Satokari Reetta R

PloS one 20130523 5

<h4>Unlabelled</h4>It is generally believed that the infant's microbiota is established during the first 1-2 years of life. However, there is scarce data on its characterization and its comparison to the adult-like microbiota in consecutive years.<h4>Aim</h4>To characterize and compare the intestinal microbiota in healthy young children (1-4 years) and healthy adults from the North Carolina region in the U.S. using high-throughput bacterial phylogenetic microarray analysis.<h4>Methods</h4>Detail ...[more]

PMID: 23717595

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Dataset Information

Intestinal microbiota in healthy U.S. young children and adults--a high throughput microarray analysis.

Unlabelled

Aim

Methods

Results

Conclusions

Publications

Intestinal microbiota in healthy U.S. young children and adults--a high throughput microarray analysis.

Similar Datasets

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