Ontology highlight
ABSTRACT:
SUBMITTER: Kantcheva AK
PROVIDER: S-EPMC3666746 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Kantcheva Adriana K AK Quick Matthias M Shi Lei L Winther Anne-Marie Lund AM Stolzenberg Sebastian S Weinstein Harel H Javitch Jonathan A JA Nissen Poul P
Proceedings of the National Academy of Sciences of the United States of America 20130502 21
Neurotransmitter:sodium symporters (NSSs) play a critical role in signaling by reuptake of neurotransmitters. Eukaryotic NSSs are chloride-dependent, whereas prokaryotic NSS homologs like LeuT are chloride-independent but contain an acidic residue (Glu290 in LeuT) at a site where eukaryotic NSSs have a serine. The LeuT-E290S mutant displays chloride-dependent activity. We show that, in LeuT-E290S cocrystallized with bromide or chloride, the anion is coordinated by side chain hydroxyls from Tyr47 ...[more]