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Development of PCR assays for detection of Trichomonas vaginalis in urine specimens.

ABSTRACT: Trichomonas vaginalis infections are usually asymptomatic or can result in nonspecific clinical symptoms, which makes laboratory-based detection of this protozoan parasite essential for diagnosis and treatment. We report the development of a battery of highly sensitive and specific PCR assays for detection of T. vaginalis in urine, a noninvasive specimen, and development of a protocol for differentiating among Trichomonas species that commonly infect humans.

SUBMITTER: Bandea CI

PROVIDER: S-EPMC3666790 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Development of PCR assays for detection of Trichomonas vaginalis in urine specimens.

Bandea Claudiu I CI Joseph Kahaliah K Secor Evan W EW Jones Laurie A LA Igietseme Joseph U JU Sautter Robert L RL Hammerschlag Margaret R MR Fajman Nancy N NN Girardet Rebecca G RG Black Carolyn M CM

Journal of clinical microbiology 20130206 4

Trichomonas vaginalis infections are usually asymptomatic or can result in nonspecific clinical symptoms, which makes laboratory-based detection of this protozoan parasite essential for diagnosis and treatment. We report the development of a battery of highly sensitive and specific PCR assays for detection of T. vaginalis in urine, a noninvasive specimen, and development of a protocol for differentiating among Trichomonas species that commonly infect humans. ...[more]

PMID: 23390274

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