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Solid-phase synthesis of C-terminal peptide libraries for studying the specificity of enzymatic protein prenylation.

ABSTRACT: Prenylation is an essential post-translational modification in all eukaryotes. Here we describe the synthesis of a 340-member library of peptides containing free C-termini on cellulose membranes. The resulting library was then used to probe the specificity of protein farnesyltransferase from S. cerevisiae.

SUBMITTER: Wang YC

PROVIDER: S-EPMC3682486 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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Solid-phase synthesis of C-terminal peptide libraries for studying the specificity of enzymatic protein prenylation.

Wang Yen-Chih YC Distefano Mark D MD

Chemical communications (Cambridge, England) 20120711 66

Prenylation is an essential post-translational modification in all eukaryotes. Here we describe the synthesis of a 340-member library of peptides containing free C-termini on cellulose membranes. The resulting library was then used to probe the specificity of protein farnesyltransferase from S. cerevisiae. ...[more]

PMID: 22783549

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