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Efficient genome editing in zebrafish using a CRISPR-Cas system.

ABSTRACT: In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.

SUBMITTER: Hwang WY

PROVIDER: S-EPMC3686313 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

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Efficient genome editing in zebrafish using a CRISPR-Cas system.

Hwang Woong Y WY Fu Yanfang Y Reyon Deepak D Maeder Morgan L ML Tsai Shengdar Q SQ Sander Jeffry D JD Peterson Randall T RT Yeh J-R Joanna JR Joung J Keith JK

Nature biotechnology 20130129 3

In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases ...[more]

PMID: 23360964

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