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KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry.

ABSTRACT: Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair, dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.

SUBMITTER: Betz K

PROVIDER: S-EPMC3690913 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry.

Betz Karin K Malyshev Denis A DA Lavergne Thomas T Welte Wolfram W Diederichs Kay K Dwyer Tammy J TJ Ordoukhanian Phillip P Romesberg Floyd E FE Marx Andreas A

Nature chemical biology 20120603 7

Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair, dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure. ...[more]

PMID: 22660438

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