Unknown

Dataset Information

0

New synthetic substrates of mammalian nucleotide excision repair system.


ABSTRACT: DNA probes for the studies of damaged strand excision during the nucleotide excision repair (NER) have been designed using the novel non-nucleosidic phosphoramidite reagents that contain N-[6-(9-antracenylcarbamoyl)hexanoyl]-3-amino-1,2-propandiol (nAnt) and N-[6-(5(6)-fluoresceinylcarbamoyl)hexanoyl]-3-amino-1,2-propandiol (nFlu) moieties. New lesion-imitating adducts being inserted into DNA show good substrate properties in NER process. Modified extended linear nFlu- and nAntr-DNA are suitable for estimation of specific excision activity catalysed with mammalian whole-cell extracts. The following substrate activity range was revealed for the model 137-bp linear double-stranded DNA: nAnt-DNA ? nFlu-DNA > Chol-DNA (Chol-DNA--legitimate NER substrate that contains non-nucleoside fragment bearing cholesterol residue). In vitro assay shows that modified DNA can be a useful tool to study NER activity in whole-cell extracts. The developed approach should be of general use for the incorporation of NER-sensitive distortions into model DNAs. The new synthetic extended linear DNA containing bulky non-nucleoside modifications will be useful for NER mechanism study and for applications.

SUBMITTER: Evdokimov A 

PROVIDER: S-EPMC3695498 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

New synthetic substrates of mammalian nucleotide excision repair system.

Evdokimov Alexey A   Petruseva Irina I   Tsidulko Aleksandra A   Koroleva Ludmila L   Serpokrylova Inna I   Silnikov Vladimir V   Lavrik Olga O  

Nucleic acids research 20130422 12


DNA probes for the studies of damaged strand excision during the nucleotide excision repair (NER) have been designed using the novel non-nucleosidic phosphoramidite reagents that contain N-[6-(9-antracenylcarbamoyl)hexanoyl]-3-amino-1,2-propandiol (nAnt) and N-[6-(5(6)-fluoresceinylcarbamoyl)hexanoyl]-3-amino-1,2-propandiol (nFlu) moieties. New lesion-imitating adducts being inserted into DNA show good substrate properties in NER process. Modified extended linear nFlu- and nAntr-DNA are suitable  ...[more]

Similar Datasets

| S-EPMC4371527 | biostudies-literature
| S-EPMC3271130 | biostudies-literature
| S-EPMC2781597 | biostudies-literature
| S-EPMC3578354 | biostudies-literature
| S-EPMC3783044 | biostudies-literature
| S-EPMC8205993 | biostudies-literature
| S-EPMC9436350 | biostudies-literature
2021-03-15 | GSE168861 | GEO
| S-EPMC85216 | biostudies-literature
| S-EPMC5656006 | biostudies-literature