Ontology highlight
ABSTRACT:
SUBMITTER: Kong M
PROVIDER: S-EPMC5656006 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Kong Muwen M Beckwitt Emily C EC Springall Luke L Kad Neil M NM Van Houten Bennett B
Methods in enzymology 20170531
Single-molecule approaches to solving biophysical problems are powerful tools that allow static and dynamic real-time observations of specific molecular interactions of interest in the absence of ensemble-averaging effects. Here, we provide detailed protocols for building an experimental system that employs atomic force microscopy and a single-molecule DNA tightrope assay based on oblique angle illumination fluorescence microscopy. Together with approaches for engineering site-specific lesions i ...[more]