Ontology highlight
ABSTRACT:
SUBMITTER: De Michele R
PROVIDER: S-EPMC3699834 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
De Michele Roberto R Ast Cindy C Loqué Dominique D Ho Cheng-Hsun CH Andrade Susana LA S Lanquar Viviane V Grossmann Guido G Gehne Sören S Kumke Michael U MU Frommer Wolf B WB
eLife 20130702
Ammonium serves as key nitrogen source and metabolic intermediate, yet excess causes toxicity. Ammonium uptake is mediated by ammonium transporters, whose regulation is poorly understood. While transport can easily be characterized in heterologous systems, measuring transporter activity in vivo remains challenging. Here we developed a simple assay for monitoring activity in vivo by inserting circularly-permutated GFP into conformation-sensitive positions of two plant and one yeast ammonium trans ...[more]