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The SxxSS motif of T-cell factor-4 isoforms modulates Wnt/?-catenin signal activation in hepatocellular carcinoma cells.

ABSTRACT: T-cell factor (TCF) proteins represent key transcription factors in Wnt signaling. We show that the SxxSS motif in TCF-4 regulates transcriptional activity in HCC cells. TCF-4K mutants increased transcriptional activity compared to TCF-4K (bearing the SxxSS); the binding pattern of co-factors in TCF-4K mutants was similar to that in TCF-4J (lacking the SxxSS). TCF activity in TCF-4K cells was suppressed by homeodomain-interacting protein kinase 2 (HIPK2), but not in TCF-4J cells. Together, our data indicates that the SxxSS motif in TCF-4K regulates transcriptional activity by modifying co-factors in the ?-catenin/TCF-4 transcriptional complex and these events may be mediated through HIPK2.

SUBMITTER: Tomimaru Y

PROVIDER: S-EPMC3700609 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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The SxxSS motif of T-cell factor-4 isoforms modulates Wnt/β-catenin signal activation in hepatocellular carcinoma cells.

Tomimaru Yoshito Y Koga Hironori H Shin Tai Ho TH Xu Chelsea Q CQ Wands Jack R JR Kim Miran M

Cancer letters 20130402 2

T-cell factor (TCF) proteins represent key transcription factors in Wnt signaling. We show that the SxxSS motif in TCF-4 regulates transcriptional activity in HCC cells. TCF-4K mutants increased transcriptional activity compared to TCF-4K (bearing the SxxSS); the binding pattern of co-factors in TCF-4K mutants was similar to that in TCF-4J (lacking the SxxSS). TCF activity in TCF-4K cells was suppressed by homeodomain-interacting protein kinase 2 (HIPK2), but not in TCF-4J cells. Together, our d ...[more]

PMID: 23562475

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