Ontology highlight
ABSTRACT:
SUBMITTER: Chou S
PROVIDER: S-EPMC3701434 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Chou Song S Flygare Johan J Lodish Harvey F HF
Experimental hematology 20130213 5
We have developed a coculture system that establishes DLK(+) fetal hepatic progenitors as the authentic supportive cells for expansion of hematopoietic stem (HSCs) and progenitor cells. In 1-week cultures supplemented with serum and supportive cytokines, both cocultured DLK(+) fetal hepatic progenitors and their conditioned medium supported rapid expansion of hematopoietic progenitors and a small increase in HSC numbers. In 2- and 3-week cultures DLK(+) cells, but not their conditioned medium, c ...[more]