Ontology highlight
ABSTRACT:
SUBMITTER: Wilkinson AC
PROVIDER: S-EPMC7206416 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Wilkinson Adam C AC Ishida Reiko R Nakauchi Hiromitsu H Yamazaki Satoshi S
Nature protocols 20200108 2
Utilizing multipotent and self-renewing capabilities, hematopoietic stem cells (HSCs) can maintain hematopoiesis throughout life. However, the mechanism behind such remarkable abilities remains undiscovered, at least in part because of the paucity of HSCs and the modest ex vivo expansion of HSCs in media that contain poorly defined albumin supplements such as bovine serum albumin. Here, we describe a simple platform for the expansion of functional mouse HSCs ex vivo for >1 month under fully defi ...[more]