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Opto-mechanical coupling in interfaces under static and propagative conditions and its biological implications.

ABSTRACT: Fluorescent dyes are vital for studying static and dynamic patterns and pattern formation in cell biology. Emission properties of the dyes incorporated in a biological interface are known to be sensitive to their local environment. We report that the fluorescence intensity of dye molecules embedded in lipid interfaces is indeed a thermodynamic observable of the system. Opto-mechanical coupling of lipid-dye system was measured as a function of the thermodynamic state of the interface. The corresponding state diagrams quantify the thermodynamic coupling between intensity I and lateral pressure ?. We further demonstrate that the coupling is conserved upon varying the temperature T. Notably, the observed opto-mechanical coupling is not limited to equilibrium conditions, but also holds for propagating pressure pulses. The non-equilibrium data show, that fluorescence is especially sensitive to dynamic changes in state such as the LE-LC phase transition. We conclude that variations in the thermodynamic state (here ? and T, in general pH, membrane potential V, etc also) of lipid membranes are capable of controlling fluorescence intensity. Therefore, interfacial thermodynamic state diagrams of I should be obtained for a proper interpretation of intensity data.

SUBMITTER: Shrivastava S

PROVIDER: S-EPMC3701664 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Opto-mechanical coupling in interfaces under static and propagative conditions and its biological implications.

Shrivastava Shamit S Shrivastava Shamit S Schneider Matthias F MF

PloS one 20130704 7

Fluorescent dyes are vital for studying static and dynamic patterns and pattern formation in cell biology. Emission properties of the dyes incorporated in a biological interface are known to be sensitive to their local environment. We report that the fluorescence intensity of dye molecules embedded in lipid interfaces is indeed a thermodynamic observable of the system. Opto-mechanical coupling of lipid-dye system was measured as a function of the thermodynamic state of the interface. The corresp ...[more]

PMID: 23861769

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Project description:BackgroundQuantification of dynamic biomechanical strain in articular cartilage in vivo; in situ using noninvasive MRI techniques is desirable and may potentially be used to assess joint pathology.PurposeTo demonstrate the use of static mechanical loading and continuous 3D-MRI acquisition of the human knee joint in vivo to measure the strain in the tibiofemoral articular cartilage.Study typeProspective.SubjectsFive healthy human volunteers (four women, one man; age 25.6?±?1.7) underwent MRI at rest, under static mechanical loading condition, and during recovery.Field strength/sequenceA field strength of 3T was used. The sequence used was 3D-continuous golden angle radial sparse parallel (GRASP) MRI and compressed sensing (CS) reconstruction.AssessmentTibiofemoral cartilage deformation maps under loading and during recovery were calculated using an optical flow algorithm. The corresponding Lagrangian strain was calculated in the articular cartilage.Statistical testsRange of displacement and strain in each subject, and the resulting mean and standard deviation, were calculated.ResultsDuring the loading condition, the cartilage displacement in the direction of loading ranged from a minimum of -673.6?±?121.9 ?m to a maximum of 726.5?±?169.5 ?m. Corresponding strain ranged from a minimum of -7.0?±?4.2% to a maximum of 5.4?±?1.6%. During the recovery condition, the cartilage displacement in the same direction reduced to a minimum of -613.0?±?129.5 ?m and a maximum of 555.7?±?311.4 ?m. The corresponding strain range reduced to a minimum of -1.6?±?7.5% to a maximum of 4.2?±?2.6%.Data conclusionThis study shows the feasibility of using static mechanical loading with continuous GRASP-MRI acquisition to measure the strain in the articular cartilage. By measuring strain during the loading and recovery phases, dynamic strain information in the articular cartilage might be able to be investigated.Level of evidence2 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2020;51:426-434.

Dataset Information

Opto-mechanical coupling in interfaces under static and propagative conditions and its biological implications.

Publications

Opto-mechanical coupling in interfaces under static and propagative conditions and its biological implications.

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