Ontology highlight
ABSTRACT:
SUBMITTER: Kassube SA
PROVIDER: S-EPMC3702667 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Kassube Susanne A SA Jinek Martin M Fang Jie J Tsutakawa Susan S Nogales Eva E
Nature structural & molecular biology 20130609 7
RECQL5 is a member of the highly conserved RecQ family of DNA helicases involved in DNA repair. RECQL5 interacts with RNA polymerase II (Pol II) and inhibits transcription of protein-encoding genes by an unknown mechanism. We show that RECQL5 contacts the Rpb1 jaw domain of Pol II at a site that overlaps with the binding site for the transcription elongation factor TFIIS. Our cryo-EM structure of elongating Pol II arrested in complex with RECQL5 shows that the RECQL5 helicase domain is positione ...[more]