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Membrane-protein binding measured with solution-phase plasmonic nanocube sensors.

ABSTRACT: We describe a solution-phase sensor of lipid-protein binding based on localized surface plasmon resonance (LSPR) of silver nanocubes. When silica-coated nanocubes are mixed in a suspension of lipid vesicles, supported membranes spontaneously assemble on their surfaces. Using a standard laboratory spectrophotometer, we calibrated the LSPR peak shift due to protein binding to the membrane surface and then characterized the lipid-binding specificity of a pleckstrin homology domain protein.

SUBMITTER: Wu HJ

PROVIDER: S-EPMC3703907 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Membrane-protein binding measured with solution-phase plasmonic nanocube sensors.

Wu Hung-Jen HJ Henzie Joel J Lin Wan-Chen WC Rhodes Christopher C Li Zhu Z Sartorel Elodie E Thorner Jeremy J Yang Peidong P Groves Jay T JT

Nature methods 20121021 12

We describe a solution-phase sensor of lipid-protein binding based on localized surface plasmon resonance (LSPR) of silver nanocubes. When silica-coated nanocubes are mixed in a suspension of lipid vesicles, supported membranes spontaneously assemble on their surfaces. Using a standard laboratory spectrophotometer, we calibrated the LSPR peak shift due to protein binding to the membrane surface and then characterized the lipid-binding specificity of a pleckstrin homology domain protein. ...[more]

PMID: 23085614

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