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Molecular and physiological analysis of three Pseudomonas aeruginosa phages belonging to the "N4-like viruses".


ABSTRACT: We present a detailed analysis of the genome architecture, structural proteome and infection-related properties of three Pseudomonas phages, designated LUZ7, LIT1 and PEV2. These podoviruses encapsulate 72.5 to 74.9 kb genomes and lyse their host after 25 min aerobic infection. PEV2 can successfully infect under anaerobic conditions, but its latent period is tripled, the lysis proceeds far slower and the burst size decreases significantly. While the overall genome structure of these phages resembles the well-studied coliphage N4, these Pseudomonas phages encode a cluster of tail genes which displays significant similarity to a Pseudomonasaeruginosa (cryptic) prophage region. Using ESI-MS/MS, these tail proteins were shown to be part of the phage particle, as well as ten other proteins including a giant 370 kDa virion RNA polymerase. These phages are the first described representatives of a novel kind of obligatory lytic P. aeruginosa-infecting phages, belonging to the widespread "N4-like viruses" genus.

SUBMITTER: Ceyssens PJ 

PROVIDER: S-EPMC3715699 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Molecular and physiological analysis of three Pseudomonas aeruginosa phages belonging to the "N4-like viruses".

Ceyssens Pieter-Jan PJ   Brabban Andrew A   Rogge Larissa L   Lewis Matthew Spooner MS   Pickard Derek D   Goulding David D   Dougan Gordon G   Noben Jean-Paul JP   Kropinski Andrew A   Kutter Elizabeth E   Lavigne Rob R  

Virology 20100901 1


We present a detailed analysis of the genome architecture, structural proteome and infection-related properties of three Pseudomonas phages, designated LUZ7, LIT1 and PEV2. These podoviruses encapsulate 72.5 to 74.9 kb genomes and lyse their host after 25 min aerobic infection. PEV2 can successfully infect under anaerobic conditions, but its latent period is tripled, the lysis proceeds far slower and the burst size decreases significantly. While the overall genome structure of these phages resem  ...[more]

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