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A chemical platform for improved induction of human iPSCs.

ABSTRACT: The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.

SUBMITTER: Lin T

PROVIDER: S-EPMC3724527 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature

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A chemical platform for improved induction of human iPSCs.

Lin Tongxiang T Ambasudhan Rajesh R Yuan Xu X Li Wenlin W Hilcove Simon S Abujarour Ramzey R Lin Xiangyi X Hahm Heung Sik HS Hao Ergeng E Hayek Alberto A Ding Sheng S

Nature methods 20091018 11

The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells. ...[more]

PMID: 19838168

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