A strategy to enhance the binding affinity of fluorophore-aptamer pairs for RNA tagging with neomycin conjugation.
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ABSTRACT:
SUBMITTER: Jeon J
PROVIDER: S-EPMC3728288 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
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A strategy to enhance the binding affinity of fluorophore-aptamer pairs for RNA tagging with neomycin conjugation.
Jeon Jongho J Lee Kyung Hyun KH Rao Jianghong J
Chemical communications (Cambridge, England) 20121001 80
Fluorogenic sulforhodamine-neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250-400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores. ...[more]
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