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Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics.

ABSTRACT: Highly functionalised ruthenium(II) tris-bipyridine receptor 1 which acts as a selective sensor for equine cytochrome c (cyt c) is shown to destabilise the native protein conformation by around 25 °C. Receptors 2 and 3 do not exert this effect confirming the behaviour is a specific effect of molecular recognition between 1 and cyt c, whilst the absence of a destabilising effect on 60% acetylated cyt c demonstrates the behaviour of 1 to be protein specific. Molecular recognition also modifies the conformational properties of the target protein at room temperature as evidenced by ion-mobility spectrometry (IMS) and accelerated trypsin proteolysis.

SUBMITTER: Wilson AJ

PROVIDER: S-EPMC3731202 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics.

Wilson Andrew J AJ Ault James R JR Filby Maria H MH Philips Hazel I A HI Ashcroft Alison E AE Fletcher Nicholas C NC

Organic & biomolecular chemistry 20130401 13

Highly functionalised ruthenium(II) tris-bipyridine receptor 1 which acts as a selective sensor for equine cytochrome c (cyt c) is shown to destabilise the native protein conformation by around 25 °C. Receptors 2 and 3 do not exert this effect confirming the behaviour is a specific effect of molecular recognition between 1 and cyt c, whilst the absence of a destabilising effect on 60% acetylated cyt c demonstrates the behaviour of 1 to be protein specific. Molecular recognition also modifies the ...[more]

PMID: 23411505

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