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Simultaneous femtosecond X-ray spectroscopy and diffraction of photosystem II at room temperature.

ABSTRACT: Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn4CaO5 cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S1) and the first illuminated state (S2) of PS II. Our simultaneous XRD-XES study shows that the PS II crystals are intact during our measurements at the LCLS, not only with respect to the structure of PS II, but also with regard to the electronic structure of the highly radiation-sensitive Mn4CaO5 cluster, opening new directions for future dynamics studies.

SUBMITTER: Kern J 

PROVIDER: S-EPMC3732582 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Simultaneous femtosecond X-ray spectroscopy and diffraction of photosystem II at room temperature.

Kern Jan J   Alonso-Mori Roberto R   Tran Rosalie R   Hattne Johan J   Gildea Richard J RJ   Echols Nathaniel N   Glöckner Carina C   Hellmich Julia J   Laksmono Hartawan H   Sierra Raymond G RG   Lassalle-Kaiser Benedikt B   Koroidov Sergey S   Lampe Alyssa A   Han Guangye G   Gul Sheraz S   Difiore Dörte D   Milathianaki Despina D   Fry Alan R AR   Miahnahri Alan A   Schafer Donald W DW   Messerschmidt Marc M   Seibert M Marvin MM   Koglin Jason E JE   Sokaras Dimosthenis D   Weng Tsu-Chien TC   Sellberg Jonas J   Latimer Matthew J MJ   Grosse-Kunstleve Ralf W RW   Zwart Petrus H PH   White William E WE   Glatzel Pieter P   Adams Paul D PD   Bogan Michael J MJ   Williams Garth J GJ   Boutet Sébastien S   Messinger Johannes J   Zouni Athina A   Sauter Nicholas K NK   Yachandra Vittal K VK   Bergmann Uwe U   Yano Junko J  

Science (New York, N.Y.) 20130214 6131

Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn4CaO5 cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S1) and the first illuminated state (S2) of PS II. Our simultaneous XRD-  ...[more]

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